determination of the median lethal dose and electrophoretic pattern of hottentotta saulcyi (scorpiones, buthidae) scorpion venom

Authors

ersenaydın yağmur alaşehir vocational school, celal bayar university, alaşehir, manisa, turkey.

özcan özkan drug and medical device agency of turkey, turkey.

kzafer karaer department of entomology and protozoology, faculty of veterinary medicine, ankara university, turkey.

abstract

background : in this study, we investigated the lethal potency, electrophoretic protein pattern and in vivo effects of hottentotta saulcyi scorpion venom in mice. methods : scorpions were collected at night, by using a uv lamp from mardin province, turkey. venom was obtained from mature h. saulcyi scorpions by electrical stimulation of the telson. the lethality of the venom was determined by i.v. injections using swiss mice. in vivo effects of the venom were assessed by using the intraperitoneal route (ip) injections into mice (20±1g) and monitored for 24 h. the protein profiles of the scorpion venom were analyzed by nupage® novex® 4–12 % gradient bis-tris gel followed by coomassie blue staining. results : the lethal assay of the venom was 0.73 mg/kg in mice. we determined the electrophoretic protein pattern of this scorpion venom to be 4, 6, 9, 31, 35, 40, 46 and 69 kda by sds-page. analysis of electrophoresis indicated that h. saulcyi scorpion intoxicated mice exhibited autonomic nervous system symptoms (tachypnea, restlessness, hyperexcitability, convulsions, salivation, lacrimation, weakness). conclusions : hottentotta saulcyi scorpion venom includes short-chain neurotoxins and long-chain neurotoxins according to the electrophoretic protein patterns. the stings of h. saulcyi scorpion must be considered of risk for humans in the southeastern region, turkey.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Determination of the Median Lethal Dose and Electrophoretic Pattern of Hottentotta saulcyi (Scorpiones, Buthidae) Scorpion Venom

BACKGROUND In this study, we investigated the lethal potency, electrophoretic protein pattern and in vivo effects of Hottentotta saulcyi scorpion venom in mice. METHODS Scorpions were collected at night, by using a UV lamp from Mardin Province, Turkey. Venom was obtained from mature H. saulcyi scorpions by electrical stimulation of the telson. The lethality of the venom was determined by i.v....

full text

Antibacterial effects of crude venom and their protein fractions of Hottentotta saulcyi scorpion

Introduction: Infectious diseases, mainly caused by bacterial agents, are one of the most common causes of death worldwide. A significant number of these agents have been resistant to one or more antibiotics; some of them are multi-drug resistant and others are extensively drug resistant. Various antimicrobial and anticancer compounds have been reported from the venom of various species of scor...

full text

Evidence for recombination in scorpion mitochondrial DNA (Scorpiones: Buthidae).

There has been very little undisputed evidence for recombination in animal mitochondrial DNA (mtDNA) provided so far. Previous unpublished results suggestive of mtDNA recombination in the scorpion family Buthidae, together with cytological evidence for a unique mechanism of mitochondrial fusion in that family, prompted us to investigate this group in more details. First, we sequenced the comple...

full text

Updated catalogue and taxonomic notes on the Old-World scorpion genus Buthus Leach, 1815 (Scorpiones, Buthidae)

Since the publication of the ground-breaking "Catalogue of the scorpions of the world (1758-1998)" (Fet et al. 2000) the number of species in the scorpion genus Buthus Leach, 1815 has increased 10-fold, and this genus is now the fourth largest within the Buthidae, with 52 valid named species. Here we revise and update the available information regarding Buthus. A new combination is proposed: Bu...

full text

the innovation of a statistical model to estimate dependable rainfall (dr) and develop it for determination and classification of drought and wet years of iran

آب حاصل از بارش منبع تأمین نیازهای بی شمار جانداران به ویژه انسان است و هرگونه کاهش در کم و کیف آن مستقیماً حیات موجودات زنده را تحت تأثیر منفی قرار می دهد. نوسان سال به سال بارش از ویژگی های اساسی و بسیار مهم بارش های سالانه ایران محسوب می شود که آثار زیان بار آن در تمام عرصه های اقتصادی، اجتماعی و حتی سیاسی- امنیتی به نحوی منعکس می شود. چون میزان آب ناشی از بارش یکی از مولفه های اصلی برنامه ...

15 صفحه اول

Robust estimation of the median lethal dose.

Alternatives to M-estimation for robust estimation of the median lethal dose in biological assays are developed. A class of link functions based on the Student-t distribution is proposed, where degrees of freedom are estimated from the data by maximum likelihood. Other alternatives include slash and finite mixture distributions. For bioassays from a pharmaceutical company, these methods extend ...

full text

My Resources

Save resource for easier access later


Journal title:
journal of arthropod-borne diseases

جلد ۹، شماره ۲، صفحات ۲۳۸-۲۴۵

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023